Application

Nuclear proteins isolated from primary neuronal cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-human Fab specific as the secondary antibody.

Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and chemiluminescent ELISA.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The conjugate is specific for human IgG and human IgG Fab fragment. The conjugate shows no reactivity with human IgG Fc fragment, mouse IgG or rat IgG.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.

Antiserum is developed in goat using purified human IgG Fab fragment as the immunogen. Antibody is isolated from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Goat anti-human IgG is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgG is determined by Enzyme Linked Immunosorbent Assay (ELISA). Cross reactivity of the antibody-conjugate is also determined by ELISA Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.

Immunogen

Developed in goat using purified human IgG Fab fragment

Other Notes

No cross-reaction with mouse and rat IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Specificity

Binds all human Igs. Provides reduced background staining with mouse or rat samples.